Regulatory
Part:BBa_K2206000:Design
Designed by: Sammy Lovat Group: iGEM17_CLSB-UK (2017-10-02)
Toehold switch for hsa-miR-15b-5p
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There must be no stop codons between the start codon and the protein coding sequence.
The RBS should not be base-paired to anything and the stop codon should have minimal base-pairing at most.
The distance between the start codon and the protein coding region has to be a multiple of three.
For more detail visit: http://2017.igem.org/Team:CLSB-UK/Design
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.